DNA

Part:BBa_J119300:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2013-05-08)


Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 906
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 907
    Illegal PstI site found at 921
    Illegal NotI site found at 7
    Illegal NotI site found at 914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 907
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 907
    Illegal PstI site found at 921
    Illegal AgeI site found at 779
    Illegal AgeI site found at 891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 129
    Illegal BsaI.rc site found at 28


Design Notes

The C-Dog parts share a -3 NT overlap with the subsequent coding sequence, making them difficult to swap as part of a library


Source

This is a place holder part only. It has no functionality other than to allow digestion with BsgI to insert other modified C-Dog parts.

References